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Junior, Valdemiro Amaro Da Silva
- Effects of Intratesticular Injection of Zinc-Based Solution in Rats in Combination With Anti-Inflammatory and Analgesic Drugs During Chemical Sterilization
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Materials and Methods: Adult rats were treated in groups: G1 (control), G2 (dimethyl sulfoxide+dipyrone); G3 (dipyrone/ zinc); G4 (dipyrone+celecoxib/zinc); G5 (dipyrone+meloxicam/zinc), and G6 (dipyrone+dexamethasone/zinc) in a single dose per day during 7 days. Animals were analyzed at 7, 15, and 30 days after treatments.
Results: The zinc-induced a widespread testicular degeneration and decreased testosterone levels even in combination with antiinflammatories or pain relievers. Testis, epididymis, prostate, and seminal vesicle had a weight reduction. The anti-inflammatory effect of dexamethasone interfered in the desired action of zinc gluconate in the 1st 15 days and celecoxib up to 7 days.
Conclusion: Meloxicam plus dipyrone did not impair the chemical sterilization based on zinc gluconate, and it can be used to reduce nociceptive effects in animals after chemical sterilization.
Authors
Simone Regina Barros De Macedo
1,
Luiz Andre Rodrigues De Lima
1,
Sandra Maria De Torres
1,
Vinicius Vasconcelos Gomes De Oliveira
1,
Rosana Nogueira De Morais
2,
Christina Alves Peixoto
3,
Bruno Mendes Tenorio
4,
Valdemiro Amaro Da Silva Junior
1
Affiliations
1 Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, BR
2 Department of Physiology, Federal University of Parana, Curitiba, Parana, BR
3 Laboratory of Ultrastructure, Aggeu Magalhaes Research Center (CPqAM), Recife, Pernambuco, BR
4 Department of Morphology, Federal University of Paraiba, Joao Pessoa, Paraiba, BR
1 Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, BR
2 Department of Physiology, Federal University of Parana, Curitiba, Parana, BR
3 Laboratory of Ultrastructure, Aggeu Magalhaes Research Center (CPqAM), Recife, Pernambuco, BR
4 Department of Morphology, Federal University of Paraiba, Joao Pessoa, Paraiba, BR
Source
Veterinary World, Vol 11, No 5 (2018), Pagination: 649-656Abstract
Aim: Chemical sterilization is a non-surgical method of contraception based on compounds injected into the testis to induce infertility. However, these injections can cause discomfort and pain able to impair the recovery of animals after this treatment. The objective of this study was to investigate if anti-inflammatories or pain relievers inhibited the sterilizing effect of zinc gluconate-based solution on the testis.Materials and Methods: Adult rats were treated in groups: G1 (control), G2 (dimethyl sulfoxide+dipyrone); G3 (dipyrone/ zinc); G4 (dipyrone+celecoxib/zinc); G5 (dipyrone+meloxicam/zinc), and G6 (dipyrone+dexamethasone/zinc) in a single dose per day during 7 days. Animals were analyzed at 7, 15, and 30 days after treatments.
Results: The zinc-induced a widespread testicular degeneration and decreased testosterone levels even in combination with antiinflammatories or pain relievers. Testis, epididymis, prostate, and seminal vesicle had a weight reduction. The anti-inflammatory effect of dexamethasone interfered in the desired action of zinc gluconate in the 1st 15 days and celecoxib up to 7 days.
Conclusion: Meloxicam plus dipyrone did not impair the chemical sterilization based on zinc gluconate, and it can be used to reduce nociceptive effects in animals after chemical sterilization.
Keywords
Analgesic, Anti-Inflammatory, Contraception, Testicular Degeneration, Zinc Gluconate.Full Text
References
- Lopez, C.A.P., Nunes-Pinheiro, D.C.S. and Figueiredo, J.R. (2005) Immunocontraception in mammals emphasizing dog population control. Rev. Bras. Reprod. Anim., 29: 159-166.
- Jana, K. and Samanta, P.K. (2011) Clinical evaluation of non-surgical sterilization of male cats with a single intra-testicular injection of calcium chloride. BMC Vet. Res., 7: 1-15.
- Fagundes, A.K., Oliveira, E.C.S., Tenorio, B.M., Melo, C.C., Nery, L.T., Santos, F.A., Alves, L.C., Douglas, R.H. and Junior, V.A.S. (2014) Injection of a chemical castration agent, zinc gluconate, into the testes of cats results in the impairment of spermatogenesis: A potentially irreversible contraceptive approach for this species? Theriogenology, 81: 230-236.
- Tepsumethanon, V., Wilde, H. and Hemachudha, T. (2005) Intratesticular injection of a balanced zinc solution for permanent sterilization of dogs. J. Med. Assoc. Thai. 88: 686-689.
- Oliveira, E.C.S., Moura, M.R., Junior, V.A.S., Peixoto, C.A., Saraiva, K.L., de Sa, M.J., Douglas, R.H. and de Pinho Marques, A.J.R. (2007) Intratesticular injection of a zinc-based solution as a contraceptive for dogs. Theriogenology, 68: 137-145.
- Bowen, R.A. (2008) Male contraceptive technology for non-human male mammals. Anim. Reprod. Sci., 105: 139-143.
- Mann, T. and Lutwak-Mann, C. (1981) Male Reproductive Function and Semen: Themes and Trends in Physiology, Biochemistry and Investigative Andrology. Springer-Verlag, New York. p495.
- Levy, J.K., Crawford, P.C., Appel, L.D. and Clifford, E.L. (2008) Comparison of intratesticular injection of zinc gluconate versus surgical castration to sterilize male dogs. Am. J. Vet. Res., 69: 140-143.
- Soto, F.R.M., Viana, W.G., Sousa, A.J., Pinheiro, S.R., Mucciolo, G.B., Hosomi, F.Y.M., Azevedo, S.S. and Dias, R.A. (2007) Evaluation of zinc gluconate, either associated or not to dimethyl sulphoxide as a contraceptive method for male dogs. Anim. Reprod., 4: 119-124.
- Wang, M. (2002) Neutersol: Intratesticular Injection Induces Sterility in Dogs. Proceedings of the International Symposium on Non-surgical Methods for Pet Population Control, Pine Mountain, Georgia. p62-65.
- Wang, M. (2004) Neutersol: From Laboratory to Market. Proceedings of the International Symposium on Non-surgical Methods for Pet Population Control, Breckenridge, Colorado. p165-169.
- Oliveira, E.C.S., Muller, P.M., Silva, F.L.M., Nery, L.T., de Sa, M.J., Guerra, M.M., Oliveira-Esquerre, K.P., Kastelic, J.P. and Douglas, R.H. (2012) Oral administration of sodium dipyrone (anti-inflammatory and analgesic) does not compromise the efficacy of intratesticular injection of zinc gluconate as a contraceptive for dogs. Anim. Reprod. Sci., 132: 207-212.
- Ortiz, M., Castaneda-Hernandeza, G. and Granados-Soto, V. (2003) Possible involvement of potassium channels in peripheral antinociception induced by metamizole: Lack of participation of ATP-sensitive K+ channels. Pharmacol. Biochem. Behav., 74: 465-470.
- Ayalasomayajula, S.P. and Kompella, U.B. (2003) Celecoxib a selective cyclooxygenase inhibitor, inhibits retinal vascular endothelial growth factor expression and vascular leakage in a streptozotocin-induced diabetic rat model. Eur. Pharmacol., 458: 283-9.
- Cupolilo, S.M.N., Gollner, A.M., Souza, R.L.P., Tiago, D.A.G., Lima, V.B.R. and Lima, T.S. (2007) Investigacao de efeitos lesivos de doses antiinflamatorias e imunossupressoras de dexametasona sobre a mucosa gastrica em camundongos. Hu. Rev., 33: 17-22.
- Bretas, F.A.V. (2014) Guia Terapeutico Veterinario. 3rd ed. Editora CEM, Rio de Jsneiro. p450.
- Fish, E.R., Brown, M.J., Danneman, P.J. and Karas, A.Z. (2008) Anaesthesia and Analgesia for Laboratory Animals. 2nd ed. Elsevier, Oxford.
- Tenorio, B.M., Jimenez, G.C., Morais, R.N., Peixoto, C.A., de Albuquerque Nogueira, R. and da Silva Junior, V.A. (2012) Evaluation of testicular degeneration induced by low-frequency electromagnetic fields. J. Appl. Toxicol., 32: 210-218.
- Siqueira Bringel, S., Amorim Junior, A.A., Amorim, M.J., Brito, L.T., Morais, R.N., de Torres, S.M., Tenorio, B.M. and Junior, V.A.S. (2013) Endocrine and testicular changes induced by olanzapine in adult Wistar rats. J. Appl. Toxicol., 33: 24-31.
- Cavalcanti, M.G.S., Filho, F.C., Mendonca, A.M., Duarte, G.R., Barbosa, C.C., De Castro, C.M., Alves, L.C. and Brayner, F.A. (2012) Morphological characterization of hemocytes from Biomphalaria glabrata and Biomphalaria straminea. Micron, 43: 285-291.
- Tenorio, B.M., Jimenez, G.C., Morais, R.N., Torres, S.M., Nogueira, R.A. and Junior, V.A.S. (2011) Testicular development evaluation in rats exposed to 60 Hz and 1 mT electromagnetic field. J. Appl. Toxicol., 31: 223-230.
- Brown, J., Walker, S.E. and Steinmain, K. (2004) Endocrine Manual for the Reproductive Assessment of Domestic and Non-domestic Species. Conservation and Research Center, Smithsonian’s National Zoological Park Front Royal, Virginia. p69.
- O’Bryan, M.K., Schlatt, S., Phillips, D.J., de Kretser, D.M. and Hedger, M.P. (2000) Bacterial lipopolysaccharide-induced inflammation compromises testicular function at multiple levels in vivo. Endocrinology, 141: 238-246.
- Reddy, M.M., Mahipal, S.V., Subhashini, J., Reddy, M.C., Roy, K.R., Reddy, G.V., Reddy, P.R. and Reddanna, P. (2006) Bacterial lipopolysaccharide-induced oxidative stress in the impairment of steroidogenesis and spermatogenesis in rats. J. Reprod. Immunol., 22: 493-500.
- Lanning, L.L., Creasy, D.M., Chapin, R.E., Mann, P.C., Barlow, N.J., Regan, K.S. and Goodman, D.G. (2002) Recommended approaches for the evaluation of testicular and epididymal toxicity. Toxicol. Pathol., 30: 507-520.
- Creasy, D.M. (2003) Evaluation of testicular toxicology: A synopsis and discussion of the recommendations proposed by the society of toxicology pathology. Birth Defects Res. B Dev. Reprod. Toxicol., 68: 408-415.
- Blazak, W.F., Treinen, K.A. and Juniewicz, P.E. (1993) Application of testicular sperm head counts in the assessment of male reproductive toxicity. In: Chapin, R.E. and Heindel, JJ., editors. Methods in Toxicology: Male Reproductive Toxicology. Academic Press, San Diego. p86-94.
- Pacheco, A. and Quirino, C.R. (2010) Sexual behavior of ram. Rev. Bra. Reprod. Anim., 34: 87-97.
- Oliveira, E.C.S., Silva, F.L.M., Muller, P.M., Brito, L.T., Fagundes, A.K.F., Sa, M.J.C., Melo, C.C.S. and Junior, V.A.S. (2011) Chemical sterilization of dogs and cats using an intratesticular injection of a zinc gluconate solution-Changing paradigms. Rev. Bras. Reprod., 35: 262-265.
- Soto, F.R.M., Viana, W.G., Mucciolo, G.C., Hosomi, F.Y., Vannucchi, C.I., Mazzei, C.P., Eyherabide, A.R., Fatima Lucio, C., Dias, R.A. and Azevedo, S.S. (2009) Evaluation of efficacy and safety of zinc gluconate associated with dimethyl sulphoxide for sexually mature canine males chemical neutering. Reprod. Domest. Anim., 44: 927-931.
- Engelhardt, G. (1996) Pharmacology of meloxicam, a new non-steroidal anti-inflammatory drug with an improved safety profile through preferential inhibition of COX-2. Br. J. Rheumatol., 35: 4-12.
- Hilario, M.O.E., Terreri, M.T. and Len, C.A. (2006) Nonsteroidal anti-inflammatory drugs: Cyclooxygenase 2 inhibitors. J. Pediatr., 82: 206-211.
- Jesus, L.E. (2000) Acute scrotum. Rev. Col. Bra. Cir., 27: 271-278.
- Grossi, G.B. (2007) Effect of submucosal injection of dexamethasone on postoperative discomfort after third molar surgery: A prospective study. J. Oral Maxillofac. Surg., 65: 2218-26.
- Santos, N.C., Figueira-Coelho, J., Martins-Silva, J. and Saldanha, C. (2003) Multidisciplinary utilization of dimethyl sulfoxide: Pharmacological, cellular, and molecular aspects. Biochem. Pharmacol. 65: 1035-1041.